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| Margot Robbie | Harley Quinn | |
| Rosie Perez | Renee Montoya | |
| Mary Elizabeth Winstead | Helena Bertinelli | |
| Jurnee Smollett | Dinah Lance | |
| Ewan McGregor | Roman Sionis | |
| Ella Jay Basco | Cassandra Cain | |
| Chris Messina | Victor Zsasz | |
| Ali Wong | Ellen Yee | |
| David Ury | Sleazy Breeder | |
| Sara Montez | Shallow Friend #1 | |
| Isabel Pakzad | Shallow Friend #2 | |
| Daniel Bernhardt | Sionis' Chauffeur | |
| Kc Strubbe | Naïve Teammate | |
| Jacky Shu | Skeptical Teammate | |
| Paloma Rabinov | Bitchy Teammate | |
| Derek Wilson | Detective Tim Monroe | |
| Joe Bucaro III | Carlo Rossi | |
| James Henry Williams Jr. | Ace Chemical Police Officer | |
| François Chau | Mr. Keo | |
| Miyuki Matsunaga | Mrs. Keo |
| Director |
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| Producer |
David Ayer
Walter Hamada Christina Hodson Geoff Johns |
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| Writer |
Christina Hodson
Paul Dini Bruce W. Timm |
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| Cinematography |
Matthew Libatique
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| Musician |
Daniel Pemberton
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Harley Quinn joins forces with a singer, an assassin and a police detective to help a young girl who had a hit placed on her after she stole a rare diamond from a crime lord. |
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